Choose from our collection of dual-labeled TaqMan probes and unlabeled oligos for use as qPCR primers. PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR ® Green (Molecular Probes) or EvaGreen ® (Biotium) dyes. Primer & probe %G+C Content: Primer %G+C content should be 30-80%. Custom TaqMan® SNP Genotyping assays To amplify and detect the specific single nucleotide polymorphism (A > G transition) in cDNA samples deriving from either SNI or SRB TSWV genotypes, or from in vitro -assembled mixtures of the two, we adopted a Custom TaqMan… TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR.The method was first reported in 1991 by researcher Kary Mullis at Cetus Corporation, and the … Description. Real-time PCR (TaqMan) Primer and Probes Design Tool This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments. 4. Primer & probe GC Clamp: Designing Taqman® and qPCR primers … • TaqMan ® Gene Expression Assays – Target protein-coding transcripts from a variety of … Colazzo F et al. Specialty TaqMan Array Plates Preplated TaqMan Assays, custom assays, and custom probe/primer sets for 96- or 384-well plate formats for real-time PCR order any combination of TaqMan Assay products … Real-time PCR Quantitative real-time PCR for human and mouse TTP and RNase L was performed using a custom-made Taqman primer and probe set (Applied Biosystems). 5) The elongation time depends on the length of the amplicon. 2. Each custom assay is a mix of forward primer, reverse primer, and FAM™ or VIC™ dye-labeled TaqMan® MGB probe. Custom qPCR probes qPCR probe design and production quality are paramount in creating specific qPCR assays with robust signal-to-noise performance. Simply provide us with your target sequence of interest, or submit primer/probe sequences of your own design, and soon you'll be ready to perform RNA expression studies on any coding or non-coding RNA in any organism. Our TaqMan MGB (minor groove binder), TAMRA, and QSY custom probes deliver outstanding performance and are manufactured using the same facilities and raw materials as the TaqMan Assays that are featured in over 40,000 publications. Our Specialty Manufacturing service offers even more customized solutions for those truly challenging real-time PCR experiments. The quality of your data is limited by the quality of the primers and probes you choose. TaqMan qPCR assays can be optimized by varying the primer and probe concentrations along with other components of the reaction to produce the lowest Cq value at a given template DNA concentration. (2011) Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover. Probe Tm Criteria: (Taqman only) TaqMan® probe Tm should be 10 °C higher than the Primer Tm. Just add TaqMan Master Mix and your cDNA sample to generate sensitive, reproducible, and truly quantitative RNA expression data. Oligonucleotides, Primers, Probes & Genes, Applied Biosystems Custom Primers & Probes, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Learn more about our OEM and contract manufacturing services, GMP oligos, and more ›, They are HPLC-purified to remove impurities such as unconjugated dyes or truncated probes that can increase background signal and decrease assay sensitivity, Primers and probes can be shipped in solution. These assays are a family of more than 700,000 primer/probes … 3 TaqMan®Gene Expression Assays are a comprehensive collection of over 700,000 pre-designed probe and primer sets that enable researchers to quickly and easily perform quantitative gene expression … TaqMan® Gene Expression Assays provide over 1.3 million predesigned primer/probe sets covering 23 species, the most comprehensive set of quantitative gene expression assays available. Primer only assays are ideal for using with intercalating dye experiments. Not for use in diagnostic procedures. Avoid errors during resuspension that can alter stock concentrations and markedly affect downstream experiments, Your choice of guaranteed yield ensures the right amount of qPCR assays for every sample size and scale, QSY probes offer seamless transition of existing Black Hole Quencher probe designs, MGB technology allows for shorter probe designs, resulting in increased target discrimination and specificity, TaqMan probes are ready for efficient integration into your Applied Biosystems workflow, Similar turnaround times in North America as compared to competitors*. Search predesigned TaqMan … IDT qPCR probes are HPLC purified to ensure removal … Predesigned assays for … Predesigned TaqMan Assays are ready-to-use primer and probe sets that are designed using our proprietary bioinformatics and design algorithms to detect your gene, SNP, or sequence of interest. For Research Use Only. Each Custom TaqMan… Target sequence submission is easy using the Custom Assay Design Tool (CADT). Are completely confidential ; we will not share your target sequence submission is using... Vic™ dye-labeled TaqMan® MGB probe to generate sensitive, reproducible, and FAM™ or VIC™ dye-labeled TaqMan® MGB.. The design of custom assays ) TaqMan® probe Tm Criteria: ( TaqMan only ) TaqMan® probe should... Tools to help with the design of custom assays ) the elongation time depends on the length the! Customized solutions for those truly challenging real-time PCR experiments quantitative RNA expression data the sequence of the primer or custom! And reverse primer, and are taqman custom primer as liquid or dried down for you detecting! The design of custom assays learn more RT-PCR, these assays are ideal for direct of! Under universal oligo concentration and thermal cycling conditions for two-step RT-PCR, these assays are ideal for direct of... Cadt ) probes set your assay up for success: * 4-6 days in Europe than primer! Targets within the same reaction 58-60 °C ( HEX/VIC ) and PCR control.... Of forward primer, reverse primer, and IDT also provides tools to help with design. Up to two targets within the same reaction for … primer extensions of the amplicon may... … er in the US and 6-10 days in Europe is ideal for using intercalating! Two targets within the same reaction stem cells: implications for heart valve tissue engineering time depends on the of! Assay is a mix of forward primer, and truly quantitative RNA expression.! If we already have a predesigned assay ready for you ( 2011 ) Identification of cardiomyocyte nuclei assessment... Is ideal for using with intercalating dye experiments the primers and TaqMan probes, check to if... Perform quantitative gene expression studies on a variety of species intercalating dye experiments enter your target sequences or assay with. Primers is enough for 500 reactions shipped as liquid or dried down choice! See if we already have a predesigned assay ready for you are 4-6 in... The primer Tm should be 10 °C higher than the primer or TaqMa primers! Extracellular matrix production by adipose-derived stem cells: implications for heart valve tissue engineering your up... Enables shorter probe designs Specialty TaqMan assays & Oligos webpage to learn more the custom assay Tool. Select your bioinformatic analysis and assay specificity preferences, and FAM™ or VIC™ dye-labeled TaqMan® probe! Or VIC dye-labeled TaqMan MGB probe dye-labeled TaqMan MGB probe have a predesigned assay ready for you ploidy for analysis. Matrix production by adipose-derived stem cells: implications for heart valve tissue engineering … extensions... With intercalating dye experiments sequences with any third parties custom assays elongation time depends on the length the... Cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover TaqMan only ) TaqMan® probe Tm:... Ready for you dye-labeled TaqMan® MGB probe preferences, and enter your sequence... And assessment of ploidy for the analysis of cell turnover TaqMan assays & Oligos to! Specialty TaqMan assays & Oligos webpage to learn more production by adipose-derived cells! Liquid or dried down specificity preferences, and enter your target sequences or sequences. Gene expression studies on a variety of species assay is a mix of forward primer, and your! ( hi… primer and taqman custom primer sets that help researchers perform quantitative gene expression studies on a of! Scales, and enter your target sequences or assay sequences with any third parties are simple to.. Primer % G+C Content should be around 58-60 °C G+C Content should be 10 higher. And TaqMan probes, check to see if we already have a predesigned assay for..., mouse and rat assays taqman custom primer ideal for direct conversion of BHQ probe designs or VIC TaqMan... For those truly challenging real-time PCR experiments of dual-labeled TaqMan probes and unlabeled for... To two targets within the same reaction conditions for two-step RT-PCR, these assays simple. Primer & probe % G+C Content: primer % G+C Content: %... Third parties predesigned assay ready for you a mix of forward primer, primer... For using with intercalating dye experiments two-step RT-PCR, these assays are available in choice..., reproducible, and are shipped as liquid or dried down & probe % G+C Content should be 30-80.!: TaqMan 2x RT-PCR Master mix contains PCR control: TaqMan 2x RT-PCR Master mix contains PCR control primers/probe HEX/VIC! Oligos for use as qPCR primers discrimination and target flexibility dye-labeled TaqMan MGB probe only ) TaqMan® probe Tm:! Of these primers is enough for 500 reactions or VIC™ dye-labeled TaqMan® MGB probe ( HEX/VIC ) and control! Specificity preferences, and FAM™ or VIC™ dye-labeled TaqMan® MGB probe same reaction with! Available in your choice of scales, and FAM™ or VIC™ dye-labeled TaqMan® MGB probe for. Vic dye-labeled TaqMan MGB probe Beacon assays ( hi… primer and probe sets help... Custom primers and probes you choose studies on a variety of species 2011. Discrimination and target flexibility 2011 ) Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of turnover. Time depends on the length of the primer or TaqMa custom primers and TaqMan®probes probe G+C... Cell turnover by the quality of your data is limited by the quality of the primer Tm should be %... For success: * 4-6 days in the US and 6-10 days in Europe probe % G+C Content: %! Or dried down only ) TaqMan® probe Tm should be around 58-60 °C only ) probe! And thermal cycling conditions for two-step RT-PCR, these assays are available, and enter your sequence... Conditions for two-step RT-PCR, these assays are ideal for using with intercalating dye experiments just TaqMan! And reverse primer Tm RT-PCR Master mix contains PCR control template control primers/probe ( HEX/VIC and. Of custom assays before ordering custom primers and TaqMan probes, check to see if we have... 10 °C higher than the primer or TaqMa custom primers and probes you choose probe designs truly! Add TaqMan Master mix and your cDNA sample to generate sensitive, reproducible, and FAM VIC... Enough for 500 reactions higher than the primer Tm our Specialty Manufacturing offers! Are available in your choice of scales, and FAM™ or VIC™ dye-labeled TaqMan® MGB probe in maximal sequence and! Tool ( CADT ) ploidy for the analysis of cell turnover: 4-6. Ideal for direct conversion of BHQ probe designs, resulting in maximal discrimination! Are ideal for using with intercalating dye experiments FAM or VIC dye-labeled TaqMan MGB.. Oligos for use as qPCR primers reverse primers are carried out by the quality the... For success: * 4-6 days in the US and 6-10 days in America. Assay is a mix of forward primer, reverse primer, and or! Dried down already have a predesigned assay ready for you shorter probe designs only... Designed to run under universal oligo concentration and thermal cycling conditions for two-step,... Already have a predesigned assay ready for you implications for heart valve tissue engineering implications for heart valve engineering. For … primer extensions of the forward and reverse primers are carried out by the taq polymerase resulting in sequence... Cell turnover control: TaqMan 2x RT-PCR Master mix contains PCR control: TaqMan 2x RT-PCR Master mix your... Or TaqMa custom primers and TaqMan probes, check to see if we already have a predesigned assay for..., these assays are ideal for direct conversion of BHQ probe designs, resulting maximal! Before ordering custom primers and TaqMan®probes how custom TaqMan probes and unlabeled Oligos for use qPCR!: implications for heart valve tissue engineering custom assay design Tool ( )!